Microbiology : Making Agar Plates.

Introduction to Microbiology class : our tutor is mr.robert, at 1400 hours, we were assigned to do a simple practical on making Agar plates. we were told to divide ourselves  into 3 groups. My practical partners for today were Amaliah and Dalilah. There were 3 types of Agar mixtures, and we chose china blue. because we were so interested with the color. LOL. ^ ^

1. Sir Robert  wrote out on the board and explained about the procedure.
2. The procedures on how to make agar plates.
3. Amaliah & Dalilah weighted the required agar powder. we only need 7g and with it we can make10 Agar plates.
4. shows the type of Agar powder we used. China Blue.

1. After we weighted the powder, we mixed it with 200ml of distilled water.
2. Then the next procedure was to heat it up, so that the mixture is fully dissolved. heat the mixture until you can see bubbles of steams.
3. next thing we did was, we poured the mixture into a bottle.
4. mixture is ready to be autoclave.

1. This  is an autoclave, Most buffers and other solutions used in any lab are sterilized before use to prevent bacterial and fungal growth during storage. we inserted the bottle of mixture into the autoclave.
2. We heated the bottle at 121 degree Celsius.
3. This is how the interior of an autoclave  looks like.
4. To prevent the solutions from boiling over/vaporizing, the autoclave chamber is pressurized during this process.

1. Dalilah inserted the bottle into the Autoclave. Make sure the cap of the bottle is loose.
2. The pressure Gauge.
3. this is when the pressure increases. the bottle have been inside the autoclave for 25 min. its time to take the bottle out.
4. the bottle is hot, so we used tap water to cool it down before we pour the agar solution into the petri dishes. but be careful, cause if you over cool it, the agar may solidifies in the bottle before you could pour it out.

1. we took 10 petri dishes and we labelled them. We used "CB" to present  "China Blue".
2. 10 petri dishes ready to be used.
3. here is where we carried out the procedure of pouring the solution into the petri dishes. 
4. petri dishes in ques ^ ^

we took turns in pouring the solution into the petri dishes.

1. the photo of other group's Agar.
2. our Agar were sealed into a clean plastic.
3. we put the sealed petri dishes into the lab fridge.

Agar is the gelatinous substance that sits inside the petri dishes used by scientists and students alike. Agar is the perfect substance for biological experiments as it holds up to bacteria and doesn't disintegrate easily. ^ ^

Sharing Biotechnology W E B S I T E with you.

Hello babes! you want to join us and be a part of Diploma in Biotechnology family?visit our new w-e-b-s-i-t-e! to know the LATEST happenings on our biotechnology course,K N O W the updated requirement to join in,stay tune and s-t-a-y u-p-d-a-t-e-d  to the department's education and social activities.
" Biotechnology,to make Bruneians life better".. InsyAllah. ^ ^ *positive aura*

http://biotechnologyofwasan.wordpress.com/ 

Everybody L O V E S Hadi

A L A I withhhhhhh H A D I

Dylaa and again with Hadi

Ameh with si Hadi Lagi! haha

LATE POST #1 Biotech BBQ day

The one whose incharge of BBQ for that day 

yummy!

can you stand looking at this pic without drooling? xp

BBQ heaven!

cherish the moment ^ ^

Yull, the youngest in my class

promoting pizza hut xp

with our group coordinator

Animal Biology Assessment round 2.

commencing the 2nd round of animal biology assessment. in order to make a good quality model,the bones are  left for one night  with peroxide. the peroxide would react immediately with the bones and after a few hours later, the bones will turn white.

with limited equipment, we had no choice but to use tooth brushes. lol.  @_@

cleaning in progress. have to clean thoroughly so  that there wouldn't be any unpleasant odor left.

the clean parts are left to air dry.

Dr.Liew made a demo on how to assemble the back bones.

 we haven't finished assembling the bones yet. with a very tight schedule, it would take a few more weeks until we could see the final outcome. so stay tune! i will post it when we're done building up the model ^ ^

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Plant pressing for Biodiversity assessment

My plant pressing on "Bunga Turi" i've been pressing the plant for  about three weeks or so and this is the outcome.
im so proud of my work xp ha ha

Second plant pressing from me. The firecracker flower. 

Building a model of chromosome.

Introduction to my chromosome model. the material used was 90% recycle materials. GO GREEN! <3

how my model looks like.... eee malu, i really am not an artistic type of person. i cant even build a simple model. haha 

labelling the sister chromatids.

100% done!

my model and dylaa's model on the right hand side. you see how creative my friend is. @_@ *envy* ha ha

this is yull's model. so purretehhh right? im sure its not easy to build up this wonderful masterpiece .

Another creative masterpiece from Muhaimin. Suhaili. bravo! ^_^

a few more creative models from my classmates.

this is one of my favorite ones. very awesome  idea to have used  bottles to present the chromosome model.

this is also one of the most purrfect model. he he i dont know how she build such purfeccttt model very creative! way to go alaina! congrats.

Animal Biology practical ROUND 1

Last year we did practical on dissecting. but don't worry, we biotech student DID NOT dissect on LIFE rabbit. In fact  we dissected a rabbit's CARCASS which have been dead because of illnesses.We need to get the bones and our assessment was to assemble the bones into a model. It was a very messy job and it took a lot of time to get the job done. but it was fun tho'. but there was also a fun spoiler.. he he.. yes, the SMELL. ha ha...it was so smelly that we had to put ointment on our nose. fuhhhh imagine that the rabbit we dissected was already dead between 2-3 months old.. Dr.Liew,our animal biology tutor, have been preserving the carcass for us in his lab freezer.. and when he defrost it, imagine the smell. hahaha. you gotta have a strong heart to do the practical.

okay, this is the part where we skinned the rabbit. we've got a female  rabbit in our hands. so before the skinning began, we observed the carcass, and learn how does a female rabbit looks like and etc. and after awhile, starts the skinning process, we cut the rabbit's ligaments 1st, as it would make it more easy and as easy as pulling a shirt off. he he i'm not kidding.

This is the part where Dr.Liew taught us about what is what, and how the organs looks like.

This is our's, skinned perfectly and ready to get the organs out.

A photo of the organs which we got the chance to see in more details. it was super awesome! and by thoroughly observing the organs,we  also found out  the possibilities of how our rabbit died.

This was the part where we finished cleaning out the organs out of the  carcass. looks a bit clean right? and the meat looked like chicken kan? hu hu




Soooo by mid day,we finished round 1 of the practical. we stopped to take our lunch, and guess what we had for lunch on that  day? boiled chicken soup, the chickens were half cooked and  reminded us of the rabbit on the last photo..we loss our appetite lah., diet for the day. hahaha

Separation of photosynthesis Pigments by Chromatography

Hello assalamualaikum... haiiii!!! today activity was fun! we just did a separation of photosynthesis pigments by chromatography in the morning... so ani gambar-gambar pagi tdi..fresh lah ni hehe. this is the first time me and dyla became partners, so far, we did okay. hehe love working with her. we get to do our part fair  and square. hehe nada yg slacking off. anywayyyy, tadi we picked  hibiscus leaves for the samples that need to be ground and used for the experiment. two of the four groups, must do the same experiment of the  same  leaves.

We use the  pastel and mortar to grind the leaves. we inserted a portion of acetone in the mortar to get the extraction of the photosynthesis pigment. and then we used a micro pippete to put the extracted ground liquid pigment onto the chromatography papers. 

Then the next procedure was to put the chromatography paper into the  solvent which dylaa have mixed earlier (where the solvent is the combination of 10% acetone and 90% of petroleum ether.)

waiting for the final outcome.  Introducing my GC Miss Hamadah.(in pink)

 so this is the results obtained from the chromatography practical we did :)

we're posing for Y O U lots of love from us ^ ^